Mechanism of neutrophils activating cGAS-STING pathway through NETs to activate NLRP3 and promote macrophage pyrosis to promote behcet disease progression

• Register: ChiCTR
• Last refreshed on: 16 August 2022
• Main ID: ChiCTR2100052808
• Date of registration: 2021-11-05
• Prospective Registration: Yes
• Primary sponsor: Huadong Hospital of Fudan University
• Public title: Mechanism of neutrophils activating cGAS-STING pathway through NETs to activate NLRP3 and promote macrophage pyrosis to promote behcet disease progression
• Scientific title: Mechanism of neutrophils activating cGAS-STING pathway through NETs to activate NLRP3 and promote macrophage pyrosis to promote behcet disease progression
• Date of first enrolment: 2022-01-01
• Target sample size: Control group:20;Active period Behcet's disease(BD) group:20;Remission period BD group:20;
• Recruitment status: Recruiting
• URL: http://www.chictr.org.cn/showproj.aspx?proj=136417
• Study type: Observational study
• Study design: Factorial
• Phase: 0

Countries of recruitment

China

Contacts

• Name: Shen Yan
• Affiliation: Huadong Hospital of Fudan University

• Name: Shen Yan
• Affiliation: Huadong Hospital of Fudan University

Key inclusion & exclusion criteria

Inclusion criteria: 1. Patients who fit the diagnosis of Behcet's disease (using the international classification criteria developed by the International Behcet Disease Study Group in 1990):
(1) Recurrent oral ulcer: doctors observed or patients complained of aphthal ulcer or scar, recurrent >= 3 times within a year;
(2) Recurrent genital ulcers: doctors have observed or patients have complained of aphthal ulcers, especially in men;
(3) Eye diseases: pre and/or post pigmenitis, cell infiltration in vitreous on slit lamp examination, or retinal vasculitis;
(4) Skin lesions: erythema nodosum lesions, pseudofolliculitis, purpuric papules, acne-like rash (without glucocorticoid);
(5) Positive acupuncture reaction: a 20 ml sterile needle was inserted diagonally into the skin, and the result was determined by the doctor 24-48 hours later;
Recurrent oral ulcers with 2 of the remaining 4 can be diagnosed as this disease;
2. Other symptoms that are closely related and helpful for diagnosis are arthralgia (arthritis).
Exclusion criteria: 1. Other diseases (infections, tumors, and other autoimmune diseases) cause symptoms similar to behcet's disease;
2. Patients with severe heart disease, cardiac insufficiency, liver dysfunction, renal insufficiency, respiratory failure, malignant tumor, gastrointestinal bleeding, etc.;
3. Mental patients;
4. Unable to take medicine on time for other reasons;
5. Pregnant and lactating women;
6. Patients who are participating in other clinical trials;
7. Clinical trial subjects who the researchers believe are not suitable for other reasons;
8. Major organ lesions: subcutaneous embolic phlebitis, deep venous embolism, arterial embolism and/or aneurysm, central neuropathy, digestive ulcer, epididymitis, and family history.

Age minimum: 18
Age maximum: 55
Gender: Both

Health Condition(s) or Problem(s) studied

Behcet's disease

Intervention(s)

Control group:No;Active period Behcet's disease(BD) group:No;Remission period BD group:No;

Primary Outcome(s)

Neutrophil extracellular traps (NETs);The expression of inflammatory factors;Infiltration of inflammatory cells;Macrophages-3;

Source(s) of Monetary Support

Huadong Hospital of Fudan University

Ethics review

Status: Approved
Approval date: 31/10/2021
Contact:

Sha Yinghao